#Minitube boar semen freezing software

The major obstacle in standardizing techniques to evaluate stallion fertility is the huge variation in ejaculates from different In conclusion, removal of SP prior to liquid storage of boar sperm for 72 h can be beneficial for their fertilizing ability. 7.16 ± 4.02 (PSP)] (P < 0.05) presented by gilts inseminated with seminal doses of ASP. This improved preservation of acrosome integrity in the absence of SP can explain the higher fertility rate (%) and number of implanted embryos at 28 days after AI (13.71 ± 4.88 (ASP) vs.

According to our results, the previous removal of SP does not affect sperm quality and the response of these cells to IVC (P > 0.05) along with resulting in a lower percentage of acrosome damage in them (P < 0.05).

In the latter, the objective was to understand how important these previous conditions can be for reproductive performance after artificial insemination (AI). The former aimed to evaluate how sperm capacity responds to in vitro capacitation (IVC) and whether their quality is affected by previous exposure to SP. From this, two experiments were conducted separately in this work: 1) in vitro and 2) in vivo assays. Thus, two treatments were prepared with semen from the sperm-rich fraction (SRF) of boar ejaculate previous to storage in liquid state: 1) PSP: semen directly extended in Beltsville Thawing Solution (BTS), and 2) ASP: semen first centrifuged with subsequent removal of supernatant (containing SP) followed by suspension of sperm in BTS.

context, the current study sought to determine the role of SP during storage of boar spermatozoa at 17 ☌ for 72 h. Under in vitro conditions, although it is generally accepted that boar semen is better preserved when SP is diluted (extended) or removed (cryopreserved), its role during storage is not completely elucidated. Seminal plasma (SP) plays a vital role in the maintenance of sperm function and integrity along with being involved in their communication with the female reproductive tract. The fertility rate was higher for the straws conserved in extender supplemented with Eugenol (80%) compared with the base extender (55.55%) and the base extender supplemented with Arginine (60%). In Eugenol supplemented extender the kinematic parameters were significantly higher for p=0.5 (30.15% progressive motility after thawing) than in the others groups (23.57%, 24.38%). Result: No significant differences were observed in samples cryopreserved in a base extender and in the base extender supplemented with Arginine. Trans-cervical artificial insemination was performed with frozen seminal material in straws. Methods: Ejaculates from Lacune rams were collected using artificial vagina, were pooled and diluted using based extender (Biladyl, Minitube) and based extender with antioxidants (Eugenol, Arginine). The purpose of this research was to evaluate the effect of two antioxidants on sperm kinematic parameters after the freezing-thawing process and the post thawed fertility rate after the trans cervical artificial insemination. Comprehensive reporting with customizable templates and the production dashboard provides extensive management control options.Backgroung: Long term storage of spermatozoa is one of the most desirable tools of assisted reproductive technologies. The product-centered approach allows for customized mapping of production processes. It stands out through its dynamic, intuitive user interface and flexible architecture.

#Minitube boar semen freezing software

This new software perfectly integrates all ejaculate data and connects all production and management processes from semen collection to reporting production statistics. Prism10 offers a modern management solution to fit your unique lab.